中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5336-5340.doi: 10.3969/j.issn. 2095-4344.2012.29. 004

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

直径可控阳极氧化铝表面有序纳米孔阵列对脐静脉内皮细胞 黏附行为的影响

倪似愚1，张燕飞1，倪世容2   

  1. 东华大学化学化工与生物工程学院，上海市 201620；
    2温州医学院病理生理教研室，浙江省温州市 325027
  • 收稿日期:2011-11-15 修回日期:2011-12-16 出版日期:2012-07-15 发布日期:2012-07-15
  • 作者简介:倪似愚☆，女，1976年生，安徽省淮南市人，汉族， 2006年中国科学院上海硅酸盐研究所毕业，博士，副研究员，主要从事生物材料的研究。 synicn@ dhu.edu.cnf

Ordered nanopore arrays on the surface of diameter-controlled anodic alumina influence the adhesion behavior of umbilical vein endothelial cells

Ni Si-yu1, Zhang Yan-fei1, Ni Shi-rong2   

  1. 1College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China;
    2Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China
  • Received:2011-11-15 Revised:2011-12-16 Online:2012-07-15 Published:2012-07-15
  • About author:Ni Si-yu☆, Doctor, Associate researcher, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China synicn@dhu.edu.cn

PDF

437

被引次数

5

摘要：

背景：研究表明多种细胞可响应材料表面微米尺度的表面结构。
目的：观察阳极氧化铝膜表面纳米孔阵列孔径变化对脐静脉内皮细胞黏附行为的影响。
方法：采用二步阳极氧化法在草酸电解液中制备出孔径可控的(25，50，65，75 nm)，高度有序排列的阳极氧化铝膜。将人脐静脉内皮细胞接种于不同孔径的阳极氧化铝膜表面，观察脐静脉内皮细胞在阳极氧化铝膜表面的黏附生长情况。
结果与结论：脐静脉内皮细胞在不同孔径阳极氧化铝表面上均良好黏附，细胞形态正常。MTT法测试结果表明培养4，24 h后，脐静脉内皮细胞在较大孔径(75 nm)阳极氧化铝膜上的黏附功能明显高于小孔径(25 nm)的阳极氧化铝膜(P < 0.05)。表明相对于小孔径(25 nm)多孔阳极氧化铝膜，较大孔径(75 nm)的多孔阳极氧化铝膜能够促进人脐静脉内皮细胞的黏附，初步显示出较好的生物学活性。

关键词: 阳极氧化铝, 表面形貌, 纳米多孔, 人脐静脉内皮细胞, 黏附

Abstract:

BACKGROUND: Studies have shown that many types of cells can influence the surface structure of micrometer scale on material surfaces.
OBJECTIVE: To observe the effect of various pore sizes of ordered nanopore arrays on diameter-controlled anodic alumina on the adhesion behavior of human umbilical vein endothelial cells (hUVECs).
METHODS: The order arranged porous anodic alumina (PAA) with controllable pore sizes (25, 50, 65 or 75 nm) was prepared using a two-step anodizing procedure in oxalic acid electrolytes. The hUVECs were seeded on the PAA surface with different pore sizes to evaluate the cellular adhesion behavior on the PAA surface.
RESULTS AND CONCLUSION: The hUVECs adhered well on the surfaces of all the PAA specimens and showed a normal morphology. MTT assay showed that the hUVECs adhesion rates were significantly higher on the surface of the PAA with larger diameter of 75 nm compared with the PAA with smaller diameter of 25 nm after 4 and 24 hours of culture (P < 0.05). It is indicated that the PAA with larger diameter of 75 nm can promote the cell adhesion compared with the PAA with smaller diameter of 25 nm based on its better biological activity.

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